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. 2015 Dec 16;5:18384. doi: 10.1038/srep18384

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Copyright © 2015, Macmillan Publishers Limited

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(A) The expression profile of JAK in shrimp tissues. The mRNA level of JAK was determined using quantitative real-time PCR. The shrimp action was used as the control. (B) The detection of JAK expression in gills of WSSV-challenged shrimp. PBS was used as a control. (C) The silencing of JAK expression in shrimp. The sequence-specific siRNA (JAK-siRNA) was injected into WSSV-infected shrimp to knock down the expression of JAK. At different time post-infection, the JAK expression was examined with quantitative real-time PCR. As a negative control, JAK-siRNA-scrambled was included in the injections. WSSV alone was used as a positive control. (D) The effect of JAK silencing on virus infection. The WSSV copies in gills of siRNA-treated shrimp were evaluated using quantitative real-time PCR. In all panels, statistically significant differences between treatments were indicated by asterisks (**p < 0.01).