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. 2015 Dec 16;5:18308. doi: 10.1038/srep18308

Figure 3. BT-061 treatment of synovial-fluid-derived mononuclear cells and whole synovial fluid induces CD4 down-modulation of T cells.

Figure 3

(A) Synovial-fluid mononuclear cells were isolated from synovial fluid of SpA patients’ joint punctuations and PBMC were isolated from the blood of the same patient. The cells were cultured for 18 h at 37 °C in wells coated with BT-061 at the indicated concentrations, in untreated wells or they were treated with soluble BT-061 before cultivation. Cells isolated from SF of three individual donors of three independent experiments are shown. From one of these donors also PBMC data are shown. (B) Statistical analysis of the percentage of gated T cells that showed CD4 down-modulation as shown in the FACS plots in (A). The error bars indicate SEM. (C) Either PBMC or synovial fluid cells from the SpA patient #3 were stained with mAbs specific for CD64 (black line) and the respective isotype control (filled grey). (D) Whole SF was incubated with 10 μg/ml BT-061 for 18 h at 37 °C and then the CD4 expression was determined cytofluometrically. During the first 4 h whole SF was steadily mixed.