Skip to main content
. 2015 Dec 16;5:18308. doi: 10.1038/srep18308

Figure 5. BT-061 treatment of tonsil derived mononuclear cells does not induce CD4 down-modulation of T cells.

Figure 5

(A) PBMC (black bars), TMC (grey bars) or CMC (white bar) were treated with the indicated concentrations of BT-061 for 45 min at 4 °C. Upon washing, the cells were cultivated for 18 h at 37 °C and then CD4 expression was determined cytofluometrically. Statistical analysis of 2 experiments with cells from 3–5 donors (PBMC), 4 experiments with cells from 6 donors (TMC) and 3 experiments with cells from 5 donors (CMC). (B) T cells isolated from PBMC or TMC derived from the same donor were cultured for 18 h at 37 °C in wells coated with BT-061 at the indicated concentrations or in untreated wells. Statistical analysis of 2 experiments with cells from 3 different donors. (C) PBMC, TMC or CMC were stained for CD64 (filled grey) and isotype control (black line). The shown histograms for PBMC are representative for data obtained by 3 experiments with cells from 3 donors and with TMC for 3 experiments with cells from 4 donors. (D) T cells isolated either from PBMC or TMC from the same donor each were treated with BT-061 or medium. Upon washing, the cells were cultured for 18 h at 37 °C in medium or together with syngeneic monocytes isolated from PBMC. Then CD4 expression was determined cytofluometrically. Representative data from 3 experiments with cell from 3 different donors are shown. (E) Statistical analysis from all experiments as shown in (D). Error bars indicate SEM.