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. 2015 Dec 16;5:18149. doi: 10.1038/srep18149

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Recombinant protein expression was induced using 0.5 mM IPTG for a cultivation time of 4 h at 37 °C. Lane M, prestained protein molecular weight marker; lane 1, crude cells extracts from E. coli BL21 star(DE3) harboring pET28a; lane 2, crude cells extracts from strain BL21/pET-mdlC; lane 3, crude cells extracts from strain BL21/pA-adhPyjhG; lane 4, crude cells extracts from strain BL21/pET-mdlC&pA-adhPyjhG; lane 5, crude cells extracts from strain BL21/pA-xylBC; lane 6, crude cells extracts from strain BL21/pE-mdlCxylBC&pA-adhPyjhG. The bands corresponding to the individual proteins were indicated by an arrow.