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. 2015 Dec 16;5:18149. doi: 10.1038/srep18149

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Copyright © 2015, Macmillan Publishers Limited

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DH5α/pTrc-mdlC, strain DH5α P. putida 2-keto acid decarboxylase; BL21/pET-mdlC&pA-yjhG, strain BL21 star(DE3) expressing P. putida 2-keto acid decarboxylase and native E. coli dehydratase; BL21/pET-mdlC&pA-adhPyjhG, strain BL21 star(DE3) expressing P. putida 2-keto acid decarboxylase and native E. coli dehydratase and aldehyde reductase. Data were obtained after each strain was induced for 48 h in liquid LB medium supplemented with 5 g/L of potassium xylonate as the substrates for BT production.