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. 2015 Dec 16;5:18149. doi: 10.1038/srep18149

Table 1. Strains and plasmids used in this study.

Strains or plasmids Genotype/Description Source
Strains
 E. coli DH5α F− recA endA1 Φ80dlacZΔM15 hsdR17(rK− mK+) λ− Invitrogen
 E. coli W3110 F− λ− rph-1 INV(rrnD rrnE) Coli Genetic Stock Center
 E. coli JM109 (DE3) recA endA1 lacZΔM15 (lac-proAB) hsdR17(rK− mK+) IDE3 Promega
 E. coli BL21 star (DE3) F− ompT hsdSB (rB− mB−) gal dcm rne131 (DE3) Invitrogen
 E. coli BL21 star (DE3) ΔxylAB Knockout of xylA and EcxylB encoding xylose isomerase and xylulose kinase 34
Plasmids
 pET28a Kanr oripBR322 lacIq T7p Novagen
 pTrcHis2B Ampr oripBR322 lacIq Trcp Invitrogen
 pETDuet-1 Ampr oripBR322 lacIq T7p Novagen
 pACYCDuet-1 Cmr oriP15A lacIq T7p Novagen
 pET-mdlC pET28a harboring P. putida mdlC This study
 pTrc-mdlC pTrcHis2B harboring P. putida mdlC This study
 pA-xylBC pACYCDuet-1 harboring C. crescentus CCxylB and xylC 34
 pE-xylB pETDuet-1 harboring C. crescentus CCxylB This study
 pE-mdlCxylB pETDuet-1 harboring P. putida mdlC and C. crescentus CCxylB This study
 pE-mdlCxylBC pETDuet-1 harboring P. putida mdlC and C. crescentus CCxylB and xylC This study
 pA-yjhG pACYCDuet-1 harboring E. coli yjhG This study
 pA-adhPyjhG pACYCDuet-1 harboring E. coli yjhG and adhP This study