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. Author manuscript; available in PMC: 2015 Dec 16.
Published in final edited form as: Toxicol In Vitro. 2012 Dec 7;27(2):721–730. doi: 10.1016/j.tiv.2012.11.017

Fig. 2.

Fig. 2

The effect of limonene and limonene + ozone reaction products on A549 cells following a 4 h exposure. A549 cells (250,000) were incubated for 24 h on transwell inserts prior to exposure. Following exposure, cells were evaluated for IL-8 and MCP-1 protein production at 12 and 24 h post-exposure. Comparisons were made for (A and B) clean air vs. limonene (20 ppm), (C and D) clean air vs. ozone (4 ppm) and (E and F) limonene (20 ppm) vs. limonene (20 ppm)/ozone (4 ppm). Bars represent the mean ± SE from three independent biological replicates per exposure group. Significant differences are designated with *p < 0.05.