Table 1.
ccpp-1 mutants display cell-specific defects in ciliary ultrastructure
| CEM Cilia | wild type | ccpp-1(ok1821) |
|---|---|---|
| MT singlets per cilium | 20 ± 3 | 16 ± 4 |
| cilium diameter (nm) | 230 ± 40 | 384 ± 45 |
| MT displacement from membrane (nm) | 25.1 ± 4.5 | 99 ± 15** |
|
Amphid Channel Cilia
| ||
| cilia per amphid channel | 10 ± 0 | 8.0 ± 1.4 |
| cilia fragments | 0 ± 0 | 3.5 ± 0.7 |
| normal doublets per cilium | 8.1 ± 0.9 | 2.0 ± 0.5** |
| central singlets | 2.4 ± 0.3 | 0.5 ± 0.2** |
We quantified characteristics of CEM cilia and amphid channel cilia middle segments (section is indicated in Fig. 3) from TEM cross-sections and tomographs. For CEM, n = 3 cilia per genotype; for amphids, n = 4 amphid channels for wild type, n = 2 amphid channels for ok8121. For number of doublets, 25 wild type and 8 ok1821 cilia had sufficient resolution in TEM or tomograph images to determine the number of normal doublets and number of singlets; values are average ± SD.
**
P < 0.01 by Mann-Whitney test.