Table 2. C. caviae phosphoproteins identified using 2D gel electrophoresis coupled with phospho-specific protein staining and MALDI-MS/MS analysis.

C. caviae GPIC				C. trachomatis			C. pneumoniae
Phosphoproteome spot identification					PD*			PD
Spot no.	Gene	Protein accession no.	Sample source†	Homologue‡	EB	RB	Homologue	EB
1, 2, 3	CCA00030§	29839798	EB		+	+		+
	(cdsD)
4	eno‖‖	29840718	EB		+	+		+
5	CCA00015¶	29839784	EB		+	+		+
7	dnaK	29840008	EB		+	+		+
8	proS	29840011	EB		+
9	groEL†	29840401	EB		+	+		+
11	sucC‖‖	29840541	EB		+			+
12	prfB#	29839933	EB		+
16	dnaB †	29839892	EB			+		+
17	glgC‖‖	29839901	EB		+
18	CCA00035 (cdsN)	29839803	EB		+			+
19, 20	pgk‖‖	29839829	EB		+			+
21	tuf†	29840456	EB		+	+		+
26	CCA00041	29839809	EB		+
28	sucB‖‖	29840183	EB		+			+
30	CCA00732	29840489	EB					+
31	hemE	29840635	EB
32	ompA	29839815	EB		+	+		+
33, 35	CCA00005	29839774	EB		+	+
	(aspC)
38	gap‖‖	29839884	EB		+	+		+
41	tal‖‖	29840447	EB		+	+		+
47, 48, 52	pyrH	29839812	EB		+			+
49	CCA00083¶	29839851	EB		+	+		+
50	tpiA‖‖	29840073	EB		+			+
56	def	29840076	EB		+	+		+
57	adk	29840294	EB		+	+		+
59	CCA00595¶	29840353	EB		+
60	aaxB	29840487	EB	P				+
61	thiE	29839970	EB	A			A
62	ssb	29840172	EB					+
63	CCA00630	29840388	EB
64	rsbV_2§	29840616	EB		+
65	CCA00330	29840094	EB
66	rsbV_1§	29840000	EB					+
5	CCA00282	29840049	RB		+	+		+
	(pmpG)
10	fusA	29839959	RB		+	+		+
15	thrS	29840712	RB		+			+
20	tkt	29840632	RB		+			+
22, 23	groEL †	29840401	RB		+	+		+
26	tig	29840674	RB		+	+		+
28, 29	atpB	29840441	RB		+			+
31	dnaB †	29839892	RB			+		+
33, 36, 37, 38	tuf †	29840456	RB		+	+		+
52	ispH	29840501	RB		+	+
53	CCA00220	29839987	RB		+	+		+

* Proteomic data reflect the positive identification of proteins from Chlamydial species in other proteomic studies. Protein source (EB versus RB) is listed where appropriate.

† Phosphoproteins identified in both EB and RB samples.

P indicates presence of a pseudogene in C. trachomatis serovars and A indicates absence of a gene homologue across Chlamydia.

§ Proteins known to be phosphorylated in Chlamydia based on previous studies.

‖‖ Proteins involved in central metabolism, phosphorylated only in EBs.

¶ Hypothetical proteins that are unique to Chlamydia.

# Protein score confidence interval of < 95 % (see Table S2).