. 2015 Aug;161(Pt 8):1659–1670. doi: 10.1099/mic.0.000118

Table 2. E. coli biomass elemental stoichiometry (C, H, N and O) and mass fraction for chemostat cultures at D = 0.4, 0.3, 0.2 or 0.1 h^− 1, under ammonium-, iron- or glucose-limited chemostat growth, and cellular glycogen content for D = 0.1 h^− 1 .

The balance of the cellular mass was assumed to be phosphorus, sulfur and ash. Degree of reduction calculated using N₂ basis. Presented means are arithmetic, unless otherwise indicated. nd, Not determined.

D (h^− 1)	n	Elemental formula	Degree of reduction	C [% ( ± sem)]^*	H [% ( ± sem)]^*	N [% ( ± sem)]^*	O [% ( ± sem)]^*	C : H	C : N	Glycogen (%)^*†
Ammonium-limited
0.4	2	CH_1.74N_0.21O_0.51	4.71 ±	45.53 ±	6.62 ±	11.28 ±	31.29 ±	0.57	4.71	nd
			0.07	0.80	0.10	0.34	2.47
0.3	2	CH_1.74N_0.21O_0.50	4.74 ±	46.73 ±	6.76 ±	11.39 ±	30.86 ±	0.58	4.79	nd
			0.01	0.54	0.02	0.18	0.39
0.2	2	CH_1.69N_0.20O_0.58	4.53 ±	44.22 ±	6.23 ±	10.12 ±	34.37 ±	0.59	5.10	nd
			0.08	0.42	0.30	0.29	4.49
0.1	3	CH_1.75N_0.19O_0.46	4.84 ±	45.66 ±	6.67 ±	10.3 ±	27.78 ±	0.57	5.17	18.2 ±
			0.10	0.76	0.24	0.27	1.50			0.5%
Mean†			4.71 ±	45.53 ±	6.57 ±	10.77 ±	31.07 ±
			0.04	0.36	0.11	0.14	1.2
Iron-limited
0.4	2	CH_1.71N_0.24O_0.43	4.85 ±	47.45 ±	6.78 ±	13.35 ±	27.28 ±	0.58	4.15	nd
			0.05	0.42	0.11	0.01	0.89
0.3	1	CH_1.69N_0.24O_0.43	4.83 ±	46.07 ±	6.47 ±	12.92 ±	26.46 ±	0.59	4.16	nd
				–	–	–	–
0.2	2	CH_1.70N_0.24O_0.40	4.9 ±	47.01 ±	6.65 ±	13.26 ±	25.13 ±	0.59	4.14	nd
			0.05	0.11	0.04	0.07	1.41
0.1	4	CH_1.71N_0.24O_0.37	4.96 ±	47.9 ±	6.83 ±	13.46 ±	23.81 ±	0.58	4.15	2.7 ±
			0.06	0.37	0.08	0.06	1.86			0.3%
Mean†			4.88 ±	47.12 ±	6.68 ±	13.25 ±	25.67 ±
			0.04	0.24	0.05	0.04	1.2
Glucose-limited
0.4	2	CH_1.74N_0.23O_0.45	4.84 ±	47.52 ±	6.89 ±	12.82 ±	28.58 ±	0.58	4.33	nd
			0.10	0.17	0.06	0.12	0.57
0.3	2	CH_1.73N_0.23O_0.42	4.88 ±	47.69 ±	6.87 ±	12.74 ±	26.93 ±	0.58	4.37	nd
			0.04	0.61	0.06	0.29	1.23
0.2	2	CH_1.69N_0.23O_0.43	4.82 ±	47.26 ±	6.67 ±	12.49 ±	27.35 ±	0.59	4.42	nd
			0.01	2.19	0.4	0.59	1.63
0.1	3	CH_1.74N_0.23O_0.43	4.88 ±	47.06 ±	6.84 ±	12.61 ±	26.97 ±	0.57	4.35	3.6 ±
			0.06	1.5	0.36	0.45	0.72			0.3%
Mean†			4.86 ±	47.38 ±	6.81 ±	12.67 ±	27.46 ±
			0.03	0.74	0.16	0.22	0.53

* Mass per cent, n = 3.

† Mean ± sem from ANOVA (least-squares).

Table 2. E. coli biomass elemental stoichiometry (C, H, N and O) and mass fraction for chemostat cultures at D = 0.4, 0.3, 0.2 or 0.1 h− 1, under ammonium-, iron- or glucose-limited chemostat growth, and cellular glycogen content for D = 0.1 h− 1 .

Table 2. E. coli biomass elemental stoichiometry (C, H, N and O) and mass fraction for chemostat cultures at D = 0.4, 0.3, 0.2 or 0.1 h^− 1, under ammonium-, iron- or glucose-limited chemostat growth, and cellular glycogen content for D = 0.1 h^− 1 .