Fig. 6. L1 protein is recruited to E1/E2 replication factories. (a) C33a cells were transfected with the Ori-containing plasmid, E1 and E2 (upper panel), and co-transfected with an L1 expression plasmid (lower panel). E2 protein was stained with sheep anti-E2 antibody (red), E1 with rabbit anti-HA antibody (green) and L1 with mouse anti-L1 antibody (magenta). DNA was stained with Hoechst (blue). (b) Cells were transfected as described for (a) and extracted with 100 mM NaCl prior to fixation as described in Methods. Images were captured by confocal microscopy. Bars, 10 μm.
