Fig. 2. Early colonization of PLNs by i.f.-inoculated MuHV-4. (a) C57BL/6 mice were infected i.f. with MHV-GFP. Sections of PLNs harvested 3 and 5 days later were stained for virus-expressed eGFP (green in merge) and for cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of at least six mice per time point. Arrowheads show examples of green/red co-localization. (b) eGFP⁺ cells were counted for five mice (three sections per mouse). Bars show means ± sem for eGFP⁺ cells expressing each marker (co-localization with ER-TR7 was < 2  %). From day 3 to day 5, eGFP⁺B220⁺ cell numbers increased significantly and eGFP⁺CD68⁺ and CD169⁺eGFP⁺ cell numbers decreased significantly (*P < 0.02).