Fig. 4. Early SCLN infection by i.n. MuHV-4 inoculation. (a) C57BL/6 mice were infected i.n. with MHV-GFP (10⁵ p.f.u.). Sections of SCLNs harvested 5 and 7 days later were stained for viral eGFP (green in merge) and cell-type markers (red in merge). Nuclei were stained with DAPI (blue). The images are representative of six mice per time point. Arrows show examples of green/red co-localization. Scale bar = 10 μm. (b) eGFP⁺ cells were counted for five mice (three sections per mouse). Bars show mean ± sem for eGFP⁺ cells expressing each marker (co-localization with ER-TR7 was < 2 %). From day 5 to day 7, eGFP⁺B220⁺ cell numbers increased significantly and eGFP⁺CD68⁺ and CD169⁺eGFP⁺ cell numbers decreased significantly (*P < 0.02).