Fig. 3. PEA-15 is involved in the evasion of FAS-mediated apoptosis and is induced by cIL-10. (a) Kasumi-3 cells were treated with control siRNA, siRNA to PEA-15 or untreated, and latency was then established for 3 days before treating with the apoptosis inducer FAS or the inhibitor of apoptosis Z-VAD-FMK as indicated and analysing for apoptosis, as described previously (Poole et al., 2011). Data represent four replicates (means ± sd). (b) The latent cells from (a) that had been treated with siRNAs to PEA-15 were harvested for Western blot analysis and probed with anti-actin (Abcam) control primary antibody or anti-PEA-15 (Abcam) antibody. (c) Primary CD34⁺ cells were either uninfected (mock) or latency was established in the presence or absence of a neutralizing anti-IL-10 antibody before harvesting and blotting for PEA-15 against a Coomassie blue loading control.