Skip to main content
. 2015 Aug;96(Pt 8):2453–2463. doi: 10.1099/vir.0.000169

Fig. 5. MiR-122 is essential for the effect of Vpr on HCV 5′ UTR activity, HCV RNA replication and HCV protein expression in the JFH1 infection model and OR6 cell lines. (a) qRT-PCR assay of the expression levels of miR-122 in miR-122-silence Huh7.5.1 cell lines and controls (miR-con). Results were normalized with respect to U6 mRNA. (b) Dual luciferase assay of p5′ UTR-luc in miR-122-silence Huh7.5.1 cell lines transfected with Vpr and controls. (c) Quantitative HCV RNA assay in miR-122-silence Huh7.5.1 cell lines transfected with Vpr and controls. (d) Western blot analysis in miR-122-silence Huh7.5.1 cell lines confirmed the expression of β-actin, NS5A and Vpr. (e) Renilla luciferase assay in miR-122-silence OR6 cell lines transfected with Vpr and controls. (f) Western blot analysis in miR-122-silence OR6 cell lines confirmed the expression of β-actin, NS5A and Vpr. The data are presented as mean ± sd. **P < 0.01, ***P < 0.001.

Fig. 5.