Table 1.
Species and culture information of Ophiocordycipitaceae used for phylogenetic analyses, and sequences of ARSEF Hirsutella cultures additionally obtained in this study, with typification of relevant taxa. Sequences obtained in this study in bold.
| GenBank accession no. | |||||||
|---|---|---|---|---|---|---|---|
| Species | Isolate no. | Host | tef1 | rpb1 | 18S rDNA | ITS rDNA1 | 28S rDNA1 |
| Hirsutella cf. haptospora | ARSEF 2228 | Diptera: Itonididae | KM652001 | KM652041 | KM652075 | KM652166 | KM652118 |
| Hirsutella citriformis | ARSEF 490 | Hemiptera: Delphacidae | KM651987 | KM652151 | KM652103 | ||
| Hirsutella citriformis | ARSEF 591 | Hemiptera: Delphacidae | KM651988 | KM652152 | KM652104 | ||
| Hirsutella citriformis | ARSEF 1035 | Hemiptera: Cixiidae | KM651989 | KM652030 | KM652064 | KM652153 | KM652105 |
| Hirsutella citriformis | ARSEF 1446 | Hemiptera: Cixiidae | KM651990 | KM652031 | KM652065 | KM652154 | KM652106 |
| Hirsutella citriformis | ARSEF 2598 | Hemiptera: Psyllidae | KM651991 | KM652155 | KM652107 | ||
| Hirsutella citriformus | ARSEF 9180 | Hemiptera: Psyllidae | KM652156 | KM652108 | |||
| Hirsutella cryptosclerotiumT | ARSEF 4517 | Hemiptera: Pseudococcidae | KM651992 | KM652032 | KM652066 | KM652157 | KM652109 |
| Hirsutella fusiformis | ARSEF 5474 | Coleoptera: Curculionidae | KM651993 | KM652033 | KM652067 | KM652110 | |
| Hirsutella gigantea | ARSEF 30 | Hymenoptera: Pamphiliidae | JX566980 | KM652034 | |||
| Hirsutella guyana | ARSEF 878 | Hemiptera: Cicadellidae | KM651994 | KM652035 | KM652068 | KM652158 | KM652111 |
| Hirsutella haptosporaT | ARSEF 2226 | Acari: Uropodina | KM651995 | KM652036 | KM652159 | ||
| Hirsutella illustris | ARSEF 5539 | Hemiptera: Aphididae | KM651996 | KM652037 | KM652069 | KM652160 | KM652112 |
| Hirsutella kirchneri | ARSEF 5551 | Acari: Eriophyidae | KM651997 | KM652070 | KM652161 | KM652113 | |
| Hirsutella lecaniicola | ARSEF 8888 | Hemiptera: Coccidae | KM651998 | KM652038 | KM652071 | KM652162 | KM652114 |
| Hirsutella liboensis | ARSEF 9603 | Lepidoptera: Cossidae | KM652072 | KM652163 | KM652115 | ||
| Hirsutella necatrixI | ARSEF 5549 | Acari | KM651999 | KM652039 | KM652073 | KM652164 | KM652116 |
| Hirsutella nodulosa | ARSEF 5473 | Lepidoptera: Pyralidae | KM652000 | KM652040 | KM652074 | KM652165 | KM652117 |
| Hirsutella radiata | ARSEF 1369 | Diptera | KM652002 | KM652042 | KM652076 | KM652119 | |
| Hirsutella repens nom. inval. | ARSEF 2348 | Hemiptera: Delphacidae | KM652003 | KM652077 | KM652167 | KM652120 | |
| Hirsutella rhossiliensis | ARSEF 2931 | Tylenchida: Heteroderidae | KM652004 | KM652043 | KM652078 | KM652168 | KM652121 |
| Hirsutella rhossiliensis | ARSEF 3207 | KM652005 | KM652044 | KM652079 | KM652169 | KM652122 | |
| Hirsutella rhossiliensis | ARSEF 3747 | Tylenchida: Criconematidae | KM652006 | KM652045 | KM652080 | KM652170 | KM652123 |
| Hirsutella rhossiliensis | ARSEF 3751 | KM652007 | KM652046 | KM652081 | KM652171 | KM652124 | |
| Hirsutella “satumaensis” | ARSEF 996 | Lepidoptera: Pyralidae | KM652008 | KM652047 | KM652082 | KM652172 | KM652125 |
| Hirsutella sinensis | ARSEF 6282 | Lepidoptera: Hepialidae | KM652009 | KM652048 | KM652083 | KM652173 | KM652126 |
| Hirsutella sp. ARSEF 7578 | ARSEF 7578 | Hymenoptera: Formicidae | JX566981 | KJ680152 | |||
| Hirsutella sp. ARSEF 8378 | ARSEF 8378 | Hemiptera: Cixiidae | KM652010 | KM652049 | KM652084 | KM652127 | |
| Hirsutella sp. NHJ | NHJ 12525 | Hemiptera | EF469063 | EF469092 | EF469125 | ||
| Hirsutella sp. OSC | OSC 128575 | Hemiptera | EF469064 | EF469093 | EF469126 | ||
| Hirsutella stilbelliformis var. myrmicarumP | IMI 396397 | Hymenoptera: Formicidae | GQ866964 | ||||
| Hirsutella strigosa | ARSEF 2044 | Hemiptera: Delphacidae | KM652011 | KM652174 | KM652128 | ||
| Hirsutella strigosa | ARSEF 2197 | Hemiptera: Cicadellidae | KM652012 | KM652050 | KM652085 | KM652175 | KM652129 |
| Hirsutella subramanianii var. myrmicarumT | IMI 396400 | Hymenoptera: Formicidae | EU797598 | ||||
| Hirsutella subulata | ARSEF 2227 | Lepidoptera: Microlepidoptea | KM652013 | KM652051 | KM652086 | KM652176 | KM652130 |
| Hirsutella thompsonii | ARSEF 241 | Acari: Eriophyidae | KM652015 | KM652178 | KM652132 | ||
| Hirsutella thompsonii | ARSEF 253 | Acari: Eriophyidae | KM652016 | KM652088 | KM652179 | KM652133 | |
| Hirsutella thompsonii | ARSEF 255 | Acari: Eriophyidae | KM652017 | KM652089 | KM652180 | KM652134 | |
| Hirsutella thompsonii | ARSEF 256 | Acari: Eriophyidae | KM652018 | KM652053 | KM652090 | KM652181 | KM652135 |
| Hirsutella thompsonii | ARSEF 257 | Acari: Eriophyidae | KM652019 | KM652054 | KM652091 | KM652182 | KM652136 |
| Hirsutella thompsonii | ARSEF 258 | Acari: Eriophyidae | KM652020 | KM652055 | KM652092 | KM652137 | |
| Hirsutella thomponii | ARSEF 259 | Acari: Eriophyidae | KM652183 | KM652138 | |||
| Hirsutella thompsonii | ARSEF 414 | Acari: Eriophyidae | KM652021 | KM652056 | KM652093 | KM652184 | KM652139 |
| Hirsutella thompsonii | ARSEF 2012 | Acari: Eriophyidae | KM652185 | KM652140 | |||
| Hirsutella thompsonii | ARSEF 2464 | Acari: Eriophyidae | KM652022 | KM652057 | KM652094 | KM652186 | KM652141 |
| Hirsutella thompsonii | ARSEF 2800 | Acari | KM652023 | KM652058 | KM652095 | KM652187 | KM652142 |
| Hirsutella thompsonii | ARSEF 3323 | Acari: Tenuipalpidae | KM652024 | KM652059 | KM652096 | KM652188 | KM652143 |
| Hirsutella thompsonii | ARSEF 3482 | KM652025 | KM652060 | KM652097 | KM652189 | KM652144 | |
| Hirsutella thompsonii | ARSEF 9457 | Acari: Tetranychidae | KM652098 | KM652190 | KM652145 | ||
| Hirsutella thompsonii “var. synnematosa” | ARSEF 1947 | Acari: Tarsonemidae | KM652026 | KM652191 | KM652146 | ||
| Hirsutella thompsonii “var. synnematosa” | ARSEF 2459 | Acari: Eriophyidae | KM652027 | KM652061 | KM652099 | KM652192 | KM652147 |
| Hirsutella thompsonii var. synnematosa | ARSEF 5412 | Acari: Tetranychidae | KM652100 | KM652193 | KM652148 | ||
| Hirsutella thompsonii var. thompsoniiN | ARSEF 137 | Acari: Eriophyidae | KM652014 | KM652052 | KM652087 | KM652177 | KM652131 |
| Hirsutella thompsonii var. vinaceaT | ARSEF 254 | Acari: Eriophyidae | KM652028 | KM652062 | KM652101 | KM652194 | KM652149 |
| Hirsutella versicolor | ARSEF 1037 | Hemiptera: Membracidae | KM652029 | KM652063 | KM652102 | KM652150 | |
| Ophiocordyceps acicularis | OSC 110987 | Coleoptera | EF468744 | EF468852 | EF468950 | ||
| Ophiocordyceps cf. acicularis | OSC 128580 | Coleoptera | DQ522326 | DQ522371 | DQ522543 | ||
| Ophiocordyceps agriotidis | ARSEF 5692 | Arthropoda | DQ522322 | DQ522368 | DQ522540 | ||
| Ophiocordyceps aphodii | ARSEF 5498 | Coleoptera: Scarabaeidae | DQ522323 | DQ522541 | |||
| Ophiocordyceps brunneipunctata | OSC 128576 | Coleoptera: Elateridae | DQ522324 | DQ522369 | DQ522542 | ||
| Ophiocordyceps clavata | NBRC 106961 | JN992461 | JN941727 | ||||
| Ophiocordyceps communis | NHJ 12581 | Isoptera | EF468775 | EF468973 | |||
| Ophiocordyceps communis | NHJ 12582 | Isoptera | EF468771 | EF468975 | |||
| Ophiocordyceps elongata | OSC 110989 | Lepidoptera | EF468748 | EF468856 | |||
| Ophiocordyceps entomorrhiza | KEW 53484 | Coleoptera | EF468749 | EF468857 | EF468954 | ||
| Ophiocordyceps gracilis | EFCC 8572 | Lepidoptera | EF468751 | EF468859 | EF468956 | ||
| Ophiocordyceps heteropoda | EFCC 10125 | Hemiptera | EF468752 | EF468860 | EF468957 | ||
| Ophiocordyceps irangiensis | OSC 128578 | Hymenoptera: Formicidae | DQ522345 | DQ522391 | DQ522556 | ||
| Ophiocordyceps irangiensis | OSC 128579 | Hymenoptera: Formicidae | EF469060 | EF469089 | EF469123 | ||
| Ophiocordyceps kniphofioides | Hymenoptera: Formicidae | KC610739 | KC610790 | ||||
| Ophiocordyceps konnoana | EFCC 7295 | Coleoptera | EF468862 | EF468958 | |||
| Ophiocordyceps konnoana | EFCC 7315 | Coleoptera | EF468753 | EF468861 | EF468959 | ||
| Ophiocordyceps longissima | EFCC 6814 | Hemiptera: Cicadidae | EF468757 | EF468865 | |||
| Ophiocordyceps melolonthae | OSC 110993 | Coleoptera: Scarabaeidae | DQ522331 | DQ522376 | DQ522548 | ||
| Ophiocordyceps myrmicarumT 2 | ARSEF 11864 | Hymenoptera: Formicidae | JX566973 | KJ680151 | KJ680150 | ||
| Ophiocordyceps nigrealla | EFCC 9247 | Lepidoptera | EF468758 | EF468866 | EF468963 | ||
| Ophiocordyceps nutans | OSC 110994 | Hemiptera: Pentatomidae | DQ522333 | DQ522378 | DQ522549 | ||
| Ophiocordyceps pruinosa | NHJ 12994 | Hemiptera | EU369024 | EU369063 | EU369106 | ||
| Ophiocordyceps pulvinata | TNS F30044 | Hymenoptera: Formicidae | GU904209 | GU904210 | GU904208 | ||
| Ophiocordyceps ravenelii | OSC 110995 | Coleoptera | DQ522334 | DQ522379 | DQ522550 | ||
| Ophiocordyceps rhizoidea | NHJ 12522 | Isoptera | EF468764 | EF468873 | EF468970 | ||
| Ophiocordyceps sinensis | EFCC 7287 | Lepidoptera | EF468767 | EF468874 | EF468971 | ||
| Ophiocordyceps sobolifera | KEW 78842 | Hemiptera: Cicadidae | EF468875 | EF468972 | |||
| Ophiocordyceps sp. OSC | OSC 110997 | Hymenoptera: Formicidae | EF468774 | EF468879 | EF468976 | ||
| Ophiocordyceps stylophora | OSC 111000 | Coleoptera: Elateridae | DQ522337 | DQ522382 | DQ522552 | ||
| Ophiocordyceps unilateralis | OSC 128574 | Hymenoptera: Formicidae | DQ522339 | DQ522385 | DQ522554 | ||
| Ophiocordyceps variabilis | ARSEF 5365 | Diptera: Xylophagidae | DQ522340 | DQ522555 | |||
| Outgroup: | |||||||
| Cordyceps gunnii | OSC 76404 | Lepidoptera | AY489616 | AY489650 | AF339572 | ||
| Nomuraea atypicola | CBS 744.73 | Arachnida | EF468786 | EF468892 | EF468987 | ||
IIndicates ex-isotype culture.
NIndicates ex-neotype culture.
PIndicated ex-paratype culture.
TIndicates ex-holotype culture.
1Sequences were not used for phylogenetic analyses.
2Ophiocordyceps myrmicarum is noted, because it is only known from its asexual life stage.