Figure 6.

Methods of the high throughput screen of small molecules. (A). Inward Tl⁺ flux in T-REx-HEK293 cells expressing AeKir1 induced by tetracycline (+ tet); data adapted from.⁵¹ (B). Inhibition of the AeKir1-mediated Tl⁺ influx by VU573 ranging in concentration from <0.3 µM to 100 µM. T-REx-HEK293 cells were pre-incubated with different concentrations of VU573 before Tl⁺ was added (arrow); data adapted from Raphemot et al.⁵³ (C). Concentration response curves of active (VU573) and inactive (VU342) small molecules obtained in Tl⁺ influx assays; the IC₅₀ is 15 µM for VU573. Data adapted from Raphemot et al.⁵³