Figure 11.

Effects of injecting VU573 and VU342 into the hemolymph of adult female yellow fever mosquitoes. A. Effects on “incapacitating” mosquitoes, i.e., rendering them flightless or dead within 24 after the hemolymph injection of VU573. Mosquitoes were injected with phosphate-buffered saline containing DMSO, the vehicle of VU small molecules, 10 mM VU573, or 10 mM VU342 (10 mM). B. Dose-response curve of the incapacitating effect of VU573 revealing an ED₅₀ of 53.6 pmol. C. Renal failure in mosquitoes induced by VU573. Urine flow per mosquito is shown. Control mosquitoes did not receive hemolymph injections. All others were injected with a K⁺ load plus DMSO (vehicle DMSO), or K⁺ load plus VU573 or VU342 in DMSO. D. K⁺ specificity of the fatal effect of VU573. Most mosquitoes survive the effects of VU573 when injected with a Na⁺ load but not with a K⁺ load. For more detail see Raphemot et al.⁵³