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. 2015 Jan 22;59(3):401–412. doi: 10.1002/mnfr.201400478

Figure 2.

Figure 2

Inhibition of VEGFR-2 activation activity of VEGF by EGCG is retained after removal of unbound polyphenol using a dialysis membrane. HUVECs were treated for 5 min with pre-incubated basal medium containing 25 ng/mL VEGF, 25 ng/mL VEGF and 1 μM EGCG, or samples prepared by dialysis (including the controls). For the dialysed samples, VEGF was incubated with or without EGCG for 30 min at room temperature prior to dialysis for 2 or 24 h at 4°C. The controls were incubated in the same conditions without dialysis. The retentate was diluted in medium to give a final concentration of 25 ng/mL VEGF and 1 μM EGCG. Phosphorylated VEGFR-2 was determined by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001 compared to their stimulated cells, respectively. Data expressed as mean ± SD (n = 3).