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. 2015 Jan 22;59(3):401–412. doi: 10.1002/mnfr.201400478

Figure 5.

Figure 5

EGCG and apple dp4 reduced binding of VEGF to endothelial cells. HUVECs in single-cell suspension in PBSA-EDTA were treated with preincubated 40 μM apple dp4 or 40 and 400 μM EGCG and 1 μg/mL biotinylated VEGF for 30 min at 4°C. Then avidin-FITC was added and the cells were incubated for a further 30 min at 4°C in the dark. Bars represent means ± SD expressed as a percentage of FITC intensity after normalisation. ***p < 0.001 compared to their respective controls. Data are expressed as mean ± SD (n = 8).