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. 2015 Nov 13;1(10):e1500723. doi: 10.1126/sciadv.1500723

Fig. 4. TSC differentiation is affected by PFI-3 inhibition.

Fig. 4

Comparison of gene expression in TSCs treated with PFI-3 or control substance during stemness and differentiation. (A to C) Differentially transcribed genes are displayed normalized and clustered to the number of reads shown as (A) tile array and (B and C) dot blots. (D) Quantification of stemness (Eomes) and differentiation markers (Prl3d1, Tpbpa) expression by RT-PCR. mRNA levels were normalized to Gapdh, Hprt, and Ppia, and values represent the means + SEM relative to the expression in TSCs treated with control compound at day 0. Experiments were independently repeated at least three times in triplicate. ***P < 0.001.