Fig. 3.

A 7-day and 14-day subculture of 8-day-old Borrelia burgdorferi stationary phase culture treated with antimicrobial agents determined by (A) SYBR Green I/PI assay and direct counting of live and dead cells stained using a mixture of SYBR Green I and propidium iodide using fluorescent microscopy. (B) Representative live/dead images of a 7-day-old subculture on stationary phase B. burgdorferi treated with antimicrobial agents taken at 200× magnification (Scale bar – 100 μm). Doxycycline (DoxC) was used as a positive control. 1× PBS and 25% alcohol were used as negative controls respectively. n = 3 ± SD, ^*p ≤ 0.05, ^**p ≤ 0.01 compared to the control. n = 3 ± SD, ⁺p ≤ 0.05, ⁺⁺p < 0.01 (doxycycline compared to Stevia A). n = 3 ± SD, ^*p ≤ 0.05, ^**p ≤ 0.01 (doxycycline compared to Stevia A). Abbreviations: doxycycline – DoxC, cefoperazone – CefP, daptomycin – DapM