Fig. 6.

Kinetics of pro-inflammatory cytokine responses in mesenetric lymphnodes of gnotobiotic TLR-4^–/– IL-10^–/– mice following A. butzleri infection. Gnotobiotic TLR-4^–/– IL-10^–/– (open symbols) and IL-10^–/– mice (closed symbols) were generated by antibiotic treatment and perorally infected either with A. butzleri (A) strain CCUG 30485 (circles) or (B) strain C1 (squares). Uninfected mice served as negative controls (Naive). IFN-γ concentrations (pg per mg tissue) were determined in supernatants of ex vivo biopsies taken from mesenterial lymphnodes (MLN) at days 6 or 16 p.i. Medians (black bars) and significance levels as determined by the Mann– Whitney U test are indicated. Significant differences between A. butzleri strains are indicated by stars within the graph (^**p < 0.01). Data shown were pooled from three independent experiments