Fig. S2.

Relative increases in intestinal and systemic IFN-γ concentrations in gnotobiotic TLR-4^–/– IL-10^–/–- mice following A. butzleri infection. Gnotobiotic TLR-4^–/– IL-10^–/– (open bars) and IL-10^–/– mice (closed bars) were generated by antibiotic treatment and perorally infected either with A. butzleri strain CCUG 30485 or strain C1 as indicated. Uninfected mice served as controls. IFN-γ concentrations were determined in supernatants of ex vivo biopsies taken from (A) ilea, (B) mesenterial lymphnodes (MLN), and (C) spleen at days 6 or 16 p.i. Bars indicate fold increases of IFN-γ levels in respective compartments upon infection. Data shown were pooled from three independent experiments