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Cytochrome c initiates caspase activation without addition of dATP or ATP. Fresh GM701 cytosol (3 μg/μl) was incubated with cytochrome c (15 μg/ml) for the time periods indicated. At the end, Western blotting was performed to detect procaspase-9 and -3 processing (A). Fifty μg of reaction mixture was also used to determine LEHDase and DEVDase activities (B). Modified from ref. 9.
