Table 2. Central composite rotatable design of KMO ester.
| Run no. | Reaction temperature, A (°C) | Reaction time, B (min) | Enzyme amount, C (wt%) | Substrate molar ratio, D (mmole) | Yield (%) | |
|---|---|---|---|---|---|---|
| Actual | Predicted | |||||
| 1 | 80.0 (0) | 240.0 (0) | 3.0 (0) | 5.0 (+2) | 1.16 | 1.00 |
| 2 | 80.0 (0) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 33.70 | 29.37 |
| 3 | 75.0 (-1) | 180.0 (-1) | 2.0 (-1) | 4.0 (+1) | 12.27 | 12.22 |
| 4 | 80.0 (0) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 34.95 | 29.37 |
| 5 | 75.0 (-1) | 180.0 (-1) | 2.0 (-1) | 2.0 (-1) | 0.25 | 1.54 |
| 7 | 85.0 (+1) | 300.0 (+1) | 4.0 (+1) | 2.0 (-1) | 5.43 | 7.28 |
| 8 | 80.0 (0) | 360.0 (+2) | 3.0 (0) | 3.0 (0) | 16.62 | 16.15 |
| 9 | 70.0 (-2) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 25.75 | 26.69 |
| 10 | 85.0 (+1) | 300.0 (+1) | 2.0 (-1) | 4.0 (+1) | 26.03 | 23.22 |
| 11 | 80.0 (0) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 30.24 | 29.37 |
| 12 | 75.0 (-1) | 300.0 (+1) | 2.0 (-1) | 2.0 (-1) | 17.20 | 15.60 |
| 14 | 75.0 (-1) | 180.0 (-1) | 4.0 (+1) | 2.0 (-1) | 5.43 | 6.22 |
| 15 | 80.0 (0) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 30.82 | 29.37 |
| 17 | 85.0 (+1) | 180.0 (-1) | 4.0 (+1) | 4.0 (+1) | 3.93 | 6.73 |
| 18 | 75.0 (-1) | 300.0 (+1) | 4.0 (+1) | 2.0 (-1) | 5.57 | 4.84 |
| 20 | 85.0 (+1) | 180.0 (-1) | 2.0 (-1) | 2.0 (-1) | 35.42 | 32.80 |
| 21 | 80.0 (0) | 120.0 (-2) | 3.0 (0) | 3.0 (0) | 5.15 | 3.47 |
| 22 | 85.0 (+1) | 180.0 (-1) | 4.0 (+1) | 2.0 (-1) | 10.26 | 8.66 |
| 23 | 75.0 (-1) | 300.0 (+1) | 2.0 (-1) | 4.0 (+1) | 24.32 | 26.28 |
| 24 | 90.0 (+2) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 27.76 | 29.05 |
| 25 | 80.0 (0) | 240.0 (0) | 5.0 (+2) | 3.0 (0) | 30.58 | 30.27 |
| 26 | 85.0 (+1) | 180.0 (-1) | 2.0 (-1) | 4.0 (+1) | 4.25 | 9.16 |
| 27 | 80.0 (0) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 22.48 | 29.37 |
| 28 | 85.0 (+1) | 300.0 (+1) | 4.0 (+1) | 4.0 (+1) | 5.69 | 5.35 |
| 29 | 80.0 (0) | 240.0 (0) | 1.0 (-2) | 3.0 (0) | 45.31 | 43.46 |
| 30 | 80.0 (0) | 240.0 (0) | 3.0 (0) | 3.0 (0) | 31.41 | 29.37 |