Arx expression is induced by FoxA2 but not by Shh signaling. Electroporation of SmoM2 (A), ΔN-Gli3 (B) and FoxA2 (C) into the chick neural tube was followed by analysis for Arx, FoxA2, Nkx2.2, Nkx6.1 and Isl2 expression. FoxA2 electroporation induced the ectopic expression of Arx, Nkx2.2, Nkx6.1 and Shh. However, electroporation of SmoM2 or ΔN-Gli3, both activators of Shh signaling induced Nkx2.2 and Nkx6.1, or Nkx6.1 and Isl2, respectively but failed to induce Arx expression. Scale bar in A is 50 μm.