Table 1. Similar effect of experimental manipulations that disrupt transport along the secretory route, or the neurofilament network, on the translocation of sAPP and ALS-relevant proteins, in CAD cell neurites.

Summary of outcome of experiments that test the translocation of AD- and ALS-relevant proteins into neurites, upon treatment of CAD cells with: BFA, BFA followed by a washout period, or peripherin-specific siRNA. Results were evaluated by comparison with non-treated CAD cells (endogenous condition, bottom row), where all tested proteins showed localization at neurite terminals (+). BFA treatment eliminates the localization of C-terminal APP epitopes (indicative of CTF), Thr⁶⁶⁸-phosphorylated CTF (pCTF), and JIP-1 (a kinesin-1 cargo, transported in association with TGN-derived vesicles). Penetration into neurites, and localization at terminals, of sAPP, FUS, TDP-43, and SOD1 is unaffected in BFA-treated cultures, but significantly diminished (downward-pointing arrows) upon transfection with peripherin-specific siRNA.

Experimental manipulation	Localization at neurite terminals
	sAPP	CTF	pCTF	JIP-1	FUS	TDP-43	SOD1
BFA	+	−	−	−	+	+	+
BFA plus washout	+	+	+	+	+	+	+
siRNA peripherin		+	+	+
Endogenous condition	+	+	+	+	+	+	+