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. Author manuscript; available in PMC: 2016 Dec 2.
Published in final edited form as: Brain Res. 2015 Oct 8;1628(0 0):254–264. doi: 10.1016/j.brainres.2015.09.033

Figure 2. Slow-migrating polyglutamine-expanded AR aggregates are less dense than fast-migrating polyglutamine-expanded AR aggregates.

Figure 2

(A) PC12 cells expressing AR10Q or AR112Q were treated with DHT and lysates were fractionated according to density on a 10–60% sucrose gradient. Slow-migrating species are retained in low-density fractions.

(B) Ten percent of each sucrose fraction was resolved by SDS-PAGE. AR112Q monomer is largely found in low-density fractions while high molecular weight species that are retained in the stacking layer of SDS-PAGE predominate in high-density fractions.