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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Nutr Neurosci. 2015 Jul 15;18(8):365–375. doi: 10.1179/1476830515Y.0000000037

Figure 1. Fetal/neonatal iron deficiency induces expression of endothelial cell markers, Glut1 and Vwf in the P10 or P12 brain.

Figure 1

Half brains, hippocampi or cerebral cortices were collected from P12 (Exp. 1, n=4–5) or P10 (Exp. 2, n=8–10) pups, total RNA was extracted, and cDNA was synthesized. Quantitative real-time PCR (qPCR) was performed for Glucose transporter 1, Glut1 (A) or von Willebrand factor, Vwf (B). Relative mRNA levels are calculated relative to an internal control cDNA sample. Data are presented as the mean ± SEM. Groups not sharing a common superscript are significantly different by one-way ANOVA and Tukey’s or Scheffe’s multiple comparison test (P < 0.05).