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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Nutr Neurosci. 2015 Jul 15;18(8):365–375. doi: 10.1179/1476830515Y.0000000037

Figure 6. Iron deficiency increases P15 brain blood vessel branching.

Figure 6

GLUT1 immunohistochemistry was performed on 40 μm sagittal brain sections from Exp. 3 P15 male pups. Confocal microscopy was used to visualize blood vessels in the control (n=6) and FeD (n=5) hippocampus and cerebral cortex. Z-stack images, with a slice interval of 1 μm, were captured through the entire 40 μm section. Representative z-projection images are shown in panels A–D. Blood vessel branching density was calculated for each treatment group in the hippocampus (E) and cerebral cortex (F). Data are presented as the mean ± SEM. Asterisks indicate a statistically significant difference by Student’s t-test (P < 0.05). DG, dentate gyrus. CA3, cornu Ammonis 3.