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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Hepatology. 2015 Jul 31;62(6):1847–1857. doi: 10.1002/hep.27939

Figure 1. Effect of RIPK1 knockdown on APAP toxicity in vivo.

Figure 1

Figure 1

Figure 1

Mice were treated with RIPK1 or Control ASO (50 mg/kg) five times, subsequently injected with APAP 300 mg/kg and euthanized at 24 hrs. (A) Serum ALT (U/L), (inset, Western blot showing protein knockdown). N=12 per group; * p value ≤ 0.05 RIPK1 vs control ASO treated mice. (B) Representative histology H&E (10x); (C) Western blot of NAPQI adducts and densitometry. N=3; (D) Mice were treated with RIPK1 or control ASO and subsequently injected with 500 mg/kg APAP and followed for 72 hrs to assess survival. Results of two independent experiments N=8 per group; RIPK1 Log Rank P. value <0.001.