Figure 2.

Effect of pressure overload on previously described mRNA targets of regulated microRNAs. RISC-bound and global mRNA levels from 1 wk TAC hearts are shown in a standardized heatmap, for microRNA-mRNA regulatory pairs described in previous reports. Red = increased mRNA abundance in RISC-bound or global fractions, blue = decreased RNA abundance. MicroRNAs observed to be regulated at 1 wk TAC in our studies¹⁴ are shown in bold, and mRNAs that exhibit predicted behavior are also shown in bold. * denotes that mRNA exhibits behavior in TAC consistent with previous report(s) but proposed regulatory microRNA does not change in the same way (e.g. miR-24-3p, Jph2). # denotes that mRNA exhibits transcript abundance consistent with previous reports in TAC, but does not appear to be dependent on proposed regulatory microRNA (Grb2, Mapk1). mRNA targets of microRNAs are drawn from the following studies: miR-1a-3p^{6, 7, 29}; miR-21-3p^{30, 31}; miR-24-3p^{32, 33}; miR-25-3p³⁴; miR-34-5p family^{9, 35}; miR-101a-3p³⁶; miR-133a-3p^{16, 20, 22}; miR-199b-5p¹¹; miR-208a-3p^{37, 38}; miR-212/132-3p¹³; miR-378-3p^{23, 24, 39}; miR-499-5p³⁸.