Figure 3. Reduced apoptosis and increased proliferation in colonic stem cells following AOM exposure.

p53^lox/lox × Lgr5^CreERT2 were injected with tamoxifen as described in Figure 1. One day later, mice were injected with AOM and terminated after 12 h (see Supplemental Figure 1A for details). A. Representative image of IHC stained apoptotic cells (red) and GFP+ stem cells (green) and nuclei (blue). Data are expressed as the percentage of GFP+ stem cells undergoing apoptosis and total apoptotic cells per crypt (mean ± SE, from 5 mice). B. Representative image of IHC stained proliferating cells (red) and GFP+ stem cells (green). Overlapping GFP+ stem cells that are proliferating appear white. Data are expressed as the percentage of GFP+ stem cells undergoing proliferation and total proliferating cells per crypt (mean ± SE, from 5 mice).