Table 1.
Tube no. | Tube | PBS (μL) | Test sample (μL) | Hydrogen peroxide (30 %) (μL) | Luminol (5 mM) (μL) |
---|---|---|---|---|---|
1-3 | Blank | 400 | – | – | |
4-6 | Negative control | 400 | – | – | 10 |
7-8 | Test | – | 400 | – | 10 |
9-11 | Positive control | 400 or semen sample | 50 | 10 |
Tube no. | Tube | PBS (μL) | Test sample (μL) | Hydrogen peroxide (30 %) (μL) | Luminol (5 mM) (μL) |
---|---|---|---|---|---|
1-3 | Blank | 400 | – | – | |
4-6 | Negative control | 400 | – | – | 10 |
7-8 | Test | – | 400 | – | 10 |
9-11 | Positive control | 400 or semen sample | 50 | 10 |