Table 1.

Set up for the measurement of ROS

Tube no.	Tube	PBS (μL)	Test sample (μL)	Hydrogen peroxide (30 %) (μL)	Luminol (5 mM) (μL)
1-3	Blank	400	–	–
4-6	Negative control	400	–	–	10
7-8	Test	–	400	–	10
9-11	Positive control	400 or semen sample		50	10