Table 2.

MCH and the raphe nuclei.

Substance	Site	Strategies	Main effect	References
MCH	DR	Microinjection and sleep recording	Increases REM sleep. Moderate increases in NREM sleep	Lagos et al., 2009
Anti-MCH antibody	DR	Microinjection and sleep recording	Decreases REM sleep. Increases wakefulness	Lagos et al., 2011a
MCH	DR	Microinjection, FST	Increases immobility time. This effect is blocked by systemic administration of fluoxetine and nortriptyline	Lagos et al., 2011b; Urbanavicius et al., 2015a
Anti-MCH antibody	DR	Microinjection, FST	Decreases immobility time	Lagos et al., 2011b
MCHR1 antagonist (ATC0175)	DR	Microinjection, FST	Reverts the pro-depressive effect of microinjections of MCH into the DR	Urbanavicius et al., 2015a
MCH	MR	Microinjection, FST	Increases immobility time	López Hill et al., 2013
MCH	DR	Unit recording, intraventricular and juxtacellular administration of MCH	Inhibit serotonergic and non-serotonergic neurons	Devera et al., 2015
MCH	DR	Microdialysis of serotonin, local perfusion of MCH	At low doses decreases serotonin release	Urbanavicius et al., 2013, 2015b

All the experiments were performed in rats. FST, forced swimming test.