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. 2015 Dec 17;6:346. doi: 10.3389/fgene.2015.00346

FIGURE 1.

FIGURE 1

Pipeline for Next-generation interaction sequencing (NGIS). Specific target binding in Y2H (or related assays) results in distinct populations of cDNAs that are identified and quantified via NGS. Interactions are scored and interpreted in a bioinformatics pipeline with quantitative statistics.