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Expression and purification of AMZ-tk from Escherichia coli. Lane M, protein marker; the molecular mass standards are indicated at the left. Lane 1, crude protein extract from non-induced cells; Lane 2, crude protein extract from IPTG-induced cells; Lane 3, soluble extract from IPTG-induced cells after heating at 70°C for 1 h; Lane 4, unbound proteins eluted from the Ni-NTA column; Lane 5, proteins eluted with 20 mM imidazole; Lane 6, proteins eluted with 300 mM imidazole.
