Table 3.

Antimicrobial activity and specificity of the AMZ-tk protease.

Microorganisms	Growth inhibition (%)	MIC value (U mL-1)
Escherichia coli	22 ± 3.5	11.5 ± 0.8
Yersinia enterocolitica	32 ± 5.2	9.2 ± 0.6
Klebesilla pneumoniae	37 ± 4.8	8.5 ± 1.1
Staphylococcus aureus	74 ± 9.2	3.1 ± 0.4
Bacillus cereus	66 ± 8.7	3.9 ± 0.4
Listeria monocytogenes	85 ± 10.6	2.1 ± 0.3

Growth inhibition and minimal inhibitory concentration (MIC) of AMZ-tk against various Gram-negative and Gram-positive bacterial strains were measured by incubation of the enzyme with the strains. The growth of the strains was recorded by spectrophotometric methods and the values were calculated as described under Section ‘Materials and Methods.’