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. 2015 Dec 14;10(12):e0144904. doi: 10.1371/journal.pone.0144904

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© 2015 Vir et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — PPD-stimulated PBMC from LTBI+ donors were co-cultured with αCD154 antibody, stained for CD69, CD4, and CD3, probed with Cy5-labeled IFNG and IL2 nucleic acid probes, and analyzed by flow cytometry. Gates were set based on unstimulated negative controls, SEB-stimulated positive controls, and FMO controls. The scatter plots show data from one donor and the pie charts from three donors. (a) Frequencies of cytokine mRNA+CD4+ T cells (left panels) and CD69 and CD154 expression in mRNA+CD4+ T cells (right panels). The upper right quadrant shows mRNA+CD69+CD154+ cells. Cytokine: IFNG (top row) and IL2 (bottom row). (b) Frequencies of all combinations of CD69 and CD154 expression in IFNG+ (top row) and IL2+ (bottom row) CD4+ T cells, one donor per column.