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. 2015 Aug 11;10(1):11–20. doi: 10.1038/ismej.2015.117

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Copyright © 2016 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Comparison of observation frequency and frequency of high abundance. Genus-level OTUs were plotted with slight transparency (alpha=0.2) such that darker points indicate more OTUs with those characteristics at that position. The OTUs were then classified as (a) Group 1: always abundant (26 samples, red), (b) Group 2: frequently abundant (⩾10 samples, yellow), (c) Group 3: transiently abundant in ⩾1 sample, green) and (d) Group 4: not abundant in any sample (gray). This was compared with the frequency at which the OTU was observed: transient (<20 samples), frequent (⩾20 samples) or core (26 samples).