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. 2015 Jul 3;10(1):210–224. doi: 10.1038/ismej.2015.83

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Copyright © 2016 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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FISH composite images (a, b) of OS filamentous streamer thin sections (5 μm) hybridized with the following DOPE probes: Cy3-Aig800 targeting Group 1A aigarchaeota (purple), Cy5-Arch915 (blue) and 6-FAM-Aqi338 (green). The aigarchaeota filaments are purple due to the overlapping emission from the Cy3 (red) and Cy5 (blue)-labeled probes. FISH composite image (c, d) with general probes Aqi338 (green), Eub338 (red) and Arch915 (blue). Scanning electron micrographs (e, f) of OS streamer community, showing filamentous organisms and condensed EPS and/or cellular appendages.