Figure 7.

The 3-hydroxypropionate/4-hydroxybutyrate CO₂ fixation cycle that is potentially operative in Calditenuis aerorheumensis (modified from Berg et al., 2007). mRNA reads from OS mapped to this pathway are reported as RPKM to take into account varying transcript lengths of the corresponding genes. Enzymes in the cycle: 1, acetyl-CoA carboxylase; 2, malonyl-CoA reductase; 3, malonate semialdehyde reductase; 4, 3-hydroxypropionyl-CoA synthetase; 5, 3-hydroxypropionyl-CoA dehydratase; 6, acryloyl-CoA reductase; 7, propionyl-CoA carboxylase; 8, methylmalonyl-CoA epimerase; 9, methylmalonyl-CoA mutase; 10, succinyl-CoA reductase; 11, succinate semialdehyde reductase; 12, 4-hydroxybutyryl-CoA synthetase; 13, 4-hydroxybutyryl-CoA dehydratase; 14, crotonyl-CoA hydratase; 15, 3-hydroxybutyryl-CoA dehydrogenase; 16, acetoacetyl-CoA β-ketothiolase. Note: 1, missing the biotin carboxylase subunit of bifunctional acetyl/propionyl-CoA carboxylase. Genes and corresponding RPKM values are presented in Supplementary Table S8.