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TAT-aequorin-based Ca2+ measurements in G. margarita germinated spores. Ca2+ assays were performed in germinated fungal spores (10 days) after 1 h incubation with 30 μM TAT-aequorin. Intracellular free Ca2+ concentration was monitored in resting conditions (a) and after treatment (arrow) with a cold shock (b) or SL GR24 (10−6 M) (c). The reported traces represent typical observed responses (n=3). Black trace, B+ line. Grey trace, cured line.
