Fig. 1.

Conformity with fiber type and cathepsin-D expression by immunohistochemical analysis in the normal soleus muscle (a–c). Transverse serial sections of normal soleus muscles stained with anti-cathepsin-D and anti-fast myosin antibodies. The immunofluorescence of cathepsin-D is shown in green (a), and that of fast myosin is shown in red (b). The left panels show merged images for cathepsin-D and fast myosin (c). Arrows indicate the same myofibers as fiber type II myofibers expressing both weak immunofluorescence reactivity for cathepsin-D and a positive reaction for fast myosin in the serial sections. Arrowheads indicate the same myofibers as type I myofibers expressing strong immunofluorescence reactivity for cathepsin-D and a negative reaction for fast myosin in the serial sections. Scale bar: 20 μm.