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. 2015 Nov 30;27(11):3585–3591. doi: 10.1589/jpts.27.3585

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2015©by the Society of Physical Therapy Science. Published by IPEC Inc.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 3. — Western blot analysis of cathepsin-D in tissue supernatant fluids from normal and degenerative soleus muscles. The three forms of cathepsin-D, which are preprocathepsin-D, procathepsin-D, and cathepsin-D mature form (heavy chain), were detected in the CON and HULT rats (a). Preprocathepsin-D level decreased in the HULT group (b). Procathepsin-D level showed no significant difference between the CON and HULT groups (c). The total inactive form of cathepsin-D, which is the amount of preprocathepsin-D and procathepsin-D, decreased in the HULT group (d), and the active form of cathepsin-D increased drastically in the HULT group (e). The results represent the mean ± SD. *Significant difference between the CON and HULT groups (p < 0.05). †Significant difference between the CON and HULT groups (p < 0.001).