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. 2015 Dec 9;6:10076. doi: 10.1038/ncomms10076

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(a–f) The M-phase-arrested cells rescued with FLAG-PCNT-Myc (WT) or FLAG-PCNT^R2231A-Myc (RA) (a–d) and FLAG-PCNT9 A-Myc (9A) (e,f) were forced to exit mitosis with ZM447439 (ZM) and co-immunostained with indicated antibodies. Scale bars, 10 μm. Centrosomal intensities of the indicated proteins were determined before and after the ZM447439 treatment and shown with the box-and-whisker plot. Centriole association was determined with the 2:1 ratio of centrin-2 and CEP135 dots in a and d–f. The centriole separation in b and c was determined, based on the proximity of centriole pairs (<1 μm). n=90 per group in 3 independent experiments. The statistical significance was determined by unpaired two-tailed t-test in Prism 6 (not significant (NS), P>0.05; *P<0.05; **P<0.01).