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. Author manuscript; available in PMC: 2015 Dec 17.
Published in final edited form as: Int J Cancer Clin Res. 2015;2(3):1–6. doi: 10.23937/2378-3419/2/4/1023

Figure 5. DIF-3(+1) inhibits breast cancer cell proliferation.

Figure 5

(A) MCF-7 and T47D cells overexpressing PAK1 were serum-deprived and incubated with vehicle (vcl), PRL (500 ng/ml) in presence or absence of IPA-3 (5μM). MCF-7 (B) or T47D (C) cells overexpressing PAK1 were serum-deprived and incubated with vehicle or 5 μM DIF-3(+1) in the presence of either vehicle, 500 ng/ml PRL, 1 nM E2, 10 ng/ml EGF or 30 ng/ml HRG. Cell density was assessed after seven days by MTT cell proliferation assay. Changes in cell numbers at day 7 are shown as percentages of the vehicle-treated cell number. Bars represent mean ± S.E., *, p <0.05 compare with cells in the same condition treated with vehicle, n = 3.