Table 1. Proteomic analysis for the binding partners of CDC42EP4 in the cerebellum.
| Identified proteins (69) | Peptide number |
Sequence coverage (%) |
||||||
|---|---|---|---|---|---|---|---|---|
| KO IgG | KO ep4 | WT IgG | WT ep4 | KO IgG | KO ep4 | WT IgG | WT ep4 | |
| CDC42EP4 | 20 | 63 | ||||||
| SEPT7 | 1 | 21 | 2.1 | 45 | ||||
| SEPT4 | 1 | 19 | 1.9 | 43 | ||||
| SEPT11 | 14 | 34 | ||||||
| SEPT5 | 12 | 42 | ||||||
| SEPT2 | 11 | 47 | ||||||
| α-II spectrin | 8 | 18 | 4.7 | 9.1 | ||||
| SEPT3 | 7 | 22 | ||||||
| Myosin-10 | 2 | 1 | 10 | 1.3 | 0.81 | 6.7 | ||
| SEPT8 | 6 | 24 | ||||||
| SEPT10 | 5 | 16 | ||||||
| SEPT6 | 5 | 26 | ||||||
| β-II spectrin | 1 | 4 | 0.8 | 2.8 | ||||
List of proteins purified from WT cerebellar lysate with CDC42EP4-immunoaffinity column and identified LC-MS/MS analysis (rightmost columns). The numbers denote the count of peptides assigned to each protein (left) and the sequence coverage (right). The blank columns are zero. The specificity of the counts is corroborated by few false-positive counts in the controls (three left columns, that is, KO lysate captured with nonimmune IgG or anti-CDC42EP4 antibody, and WT lysate captured with nonimmune IgG). Another co-IP method used for the immunoblot analyses detected GLAST (peptide number=2, sequence coverage=6.45), but not CDC42. Co-IP, Co-immunoprecipitation; KO, knockout; WT, wild type.