Table 2. Refinement and model statistics for atomic models built de novo into eVLP and CPMV-B EM density.
| eVLP | CPMV-B | |
|---|---|---|
| Data collection | ||
| Particles | 4,998 | 4,331 |
| Pixel size (Å per pixel) | 1.04 | 1.04 |
| Defocus range (μm) | 0.5–5.0 | 0.5–8.0 |
| Voltage (kV) | 300 | 300 |
| Dose (e- per Å2) | 45 | 45 |
| EM refinement | ||
| Final resolution (Å) | 3.04 | 3.44 |
| Experimental B-factor (Å2) | −74.6 | −107.6 |
| EMDB accession number | EMD-3014 | EMD-3013 |
| r.m.s. deviations | ||
| Bonds (Å) | 0.0077 | 0.0108 |
| Angles (°) | 1.2957 | 1.5319 |
| Model validation | ||
| R factor | 0.2207 | 0.2909 |
| Molprobity score | 1.61 (100th percentile) | 1.76 (100th percentile) |
| Clashscore (all atoms) | 0.81 (100th percentile) | 1.52 (100th percentile) |
| Good rotamers (%) | 96.3% | 96.5% |
| PDB ID | 5a33 | 5a32 |
EM, electron microscopy; eVLP, empty virus-like particle; CPMV-B, cowpea mosaic virus B; r.m.s., root mean squared.